Association between cyclin D1 G870A polymorphism and breast cancer susceptibility of Uygur and Han nationalities in Xinjiang

doi:10.3877/cma. j. issn.1674-0807.2015.05.003

Abstract

Abstract:

Objective

To investigate the distribution of single nucleotide polymorphism (SNP) at 870 position of exon 4 in cyclin D1 gene (CCND1)and its relationship with susceptibility to breast cancer in patients of Uygur and Han nationalities.

Methods

According to the inclusion and exclusion criteria, we enrolled 192 breast cancer patients of Uygur nationality, 200 Uygur healthy controls, 254 breast cancer patients of Han nationality and 200 Han healthy controls, who all were treated in Tumor Hospital of Xinjiang Medical University from January 2012 to December 2014, for a prospective study. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was used to detect the G870A polymorphism of CCND1 gene.CCND1 G870A genotype and allele frequency were compared by χ² test. The relationship between this polymorphism and breast cancer susceptibility in Uygur or Han population in Xinjiang was analyzed using nonconditional Logistic regression.

Results

The distribution of GG, AG, AA genotypes of CCND1 gene G870A and G/A allele frequency showed no significant difference between Uygur breast cancer patients and Uygur healthy controls [GG, AG, AA genotypes: 18.7% (36/192) vs 16.0% (32/200), 52.1%(100/192) vs 49.5% (99/200), 29.2% (56/192) vs 34.5% (69/200), χ² =1.430, P=0.489; G/A allele frequency:44.8% (172/384) vs 40.8% (163/400),55.2% (212/384) vs 59.2% (237/400), χ²=1.308, P=0.253]. The comparison between Han breast cancer patients and Han healthy controls showed the similar results [GG, AG, AA genotypes: 20.1% (51/254) vs 16.0% (32/200), 50.0% (127/254) vs 56.0% (112/200),29.9% (76/254) vs 28.0% (56/200), χ²=1.925, P=0.382; G/A allele frequency:45.1% (229/508) vs 44.0% (176/400), 54.9% (279/508) vs 56.0% (224/400), χ² =0.105, P=0.745]. Whether Han or Uighur population, the differences in the genotype distribution (χ² =0.213,P=0.899;χ²=0.007,P=0.932) and allele frequency (χ²=2.153,P=0.341;χ²=0.865,P=0.352) showed no significant difference between Uygur and Han breast cancer patients or between Uygur and Han healthy controls.Our finding showed no increased risk of breast cancer in the population carrying A allele in Uygur or Han population (A allele vs G allele: OR=0.848,95%CI 0.639-1.125, P=0.253; OR=0.957,95%CI 0.735-1.246, P=0.745).

Conclusion

There is no significant association between CCND1 G870A polymorphism and breast cancer susceptibility in Uygur or Han population in Xinjiang.

Key words: Breast neoplasms, Cyclin D1, Polymorphism, genetic, Uygur nationality

Wei Liu, Shan Li, Shun'e Yang. Association between cyclin D1 G870A polymorphism and breast cancer susceptibility of Uygur and Han nationalities in Xinjiang[J]. Chinese Journal of Breast Disease(Electronic Edition), 2015, 09(05): 298-302.

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References 17

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组别	例数	基因型[例(%)]			等位基因[个(频率)]
组别	例数	GG	AG	AA	G^a	A^b
维吾尔族
乳腺癌组	192	36(18.7)	100(52.1)	56(29.2)	172(44.8)	212(55.2)
健康对照组	200	32(16.0)	99(49.5)	69(34.5)	163(40.8)	237(59.2)
汉族
乳腺癌组	254	51(20.1)	127(50.0)	76(29.9)	229(45.1)	279(54.9)
健康对照组	200	32(16.0)	112(56.0)	56(28.0)	176(44.0)	224(56.0)

组别	例数	基因型[例(%)]			等位基因[个(频率)]
组别	例数	GG	AG	AA	G^a	A^b
维吾尔族
乳腺癌组	192	36(18.7)	100(52.1)	56(29.2)	172(44.8)	212(55.2)
健康对照组	200	32(16.0)	99(49.5)	69(34.5)	163(40.8)	237(59.2)
汉族
乳腺癌组	254	51(20.1)	127(50.0)	76(29.9)	229(45.1)	279(54.9)
健康对照组	200	32(16.0)	112(56.0)	56(28.0)	176(44.0)	224(56.0)

基因	回归系数	标准误	Word值	P值	OR值	95%置信区间
基因型
GG					1
AG	-0.108	0.281	0.147	0.702	0.898	0.517～1.558
AA	-0.327	0.302	1.167	0.280	0.721	0.399～1.305
AG+AA	-0.192	0.267	0.516	0.473	0.825	0.489～1.393
等位基因
G					1
A	-0.165	0.145	1.307	0.253	0.848	0.639～1.125

基因	回归系数	标准误	Word值	P值	OR值	95%置信区间
基因型
GG					1
AG	-0.108	0.281	0.147	0.702	0.898	0.517～1.558
AA	-0.327	0.302	1.167	0.280	0.721	0.399～1.305
AG+AA	-0.192	0.267	0.516	0.473	0.825	0.489～1.393
等位基因
G					1
A	-0.165	0.145	1.307	0.253	0.848	0.639～1.125

基因	回归系数	标准误	Word值	P值	OR值	95%置信区间
基因型
GG					1
AG	-0.340	0.260	1.713	0.191	0.711	0.427～1.185
AA	-0.161	0.286	0.315	0.574	0.852	0.486～1.492
AG+AA	-0.277	0.226	4.272	0.265	0.758	0.466～1.234
等位基因
G					1
A	-0.044	0.135	0.105	0.745	0.957	0.735～1.246

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